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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 May 11;12(8):2017–2028. doi: 10.1111/j.1600-6143.2012.04073.x

Figure 4. Increased donor-reactive T cell response in B6.CCR5−/− recipients and CD8−/−/CCR5−/− recipients.

Figure 4

Groups of wild-type C57BL/6, B6.CCR5−/− and B6.CD8−/−/CCR5−/− mice (n = 3/each group) received B6.Kd heart allografts. On day 10 post-transplant (A) whole spleen cell suspensions or (B) CD8 T cells enriched from recipient spleens by negative selection were tested by ELISPOT assay to enumerate cells producing IFN-γ and IL-4. Data are representative of two independent experiments and the data indicate mean number of donor-reactive T cells or CD8 T cells producing the test cytokine ± SEM. (A) **p =0.004 vs. wild-type C57BL/6 recipients and p = 0.009 vs. B6.CD8−/−/CCR5−/− recipients; *p = 0.012 vs. CCR5−/− recipients; (B) **p = 0.003 vs. wild-type C57BL/6 recipients.