Table 1.

List of immunological reagents, source and concentration

Reagent/antibody	Source/Cat. No.	Concentration	Buffer/pH 7.4
Normal horse serum	Vector Labs –Peterborough, UK S2000	1% or 10%	0.3% Triton X-100-TBS
Normal goat serum	Vector Labs –Peterborough, UK S1000	1% or 10%	0.3% Triton X-100-TBS
Vectastain ABC Kit	Vector Labs –Peterborough, UK PK-6100	As per manufacturer instructions	TBS
ExtrAvidin–HRP	Sigma –Dorset, UK E2886	1:1500	TBS
VIP Peroxidase substrate kit	Vector Labs –Peterborough, UK SK-4600	1:3	TBS
DAB Peroxidase substrate kit	Vector Labs –Peterborough, UK SK-4100	1:3	TBS
Avidin–Biotin blocking kit	Vector Labs –Peterborough, UK SP-2001		TBS
Biotin dextran amine 10K	Molecular Probes –Paisley, UK D1956	10%	10 mM phosphate buffer
Fluorogold	Fluorochrome –Denver, Colorado, USA LLC	2%	0.9% NaCl
Cholera toxin B subunit	List Biologicals –Campbell, California, USA 103B	5%	0.1 M PBS
Guinea pig anti GABA	ABcam –Cambridge, UK ab17413	1:700	1% NGS–0.3% Triton X-100-TBS
Guinea pig anti vGLUT2	Millipore –Oxford, UK MAB5504	1:12,000	1% NGS–0.3% Triton X-100-TBS
Rabbit anti nNOS	Santa Cruz –Santa Cruz, California, USA SC-648	1:400	1% NGS–0.3% Triton X-100-TBS
Goat anti CTB	List Biologicals –Campbell, California, USA 703	1:200,000	1% NHS–0.3% Triton X-100-TBS
Biotinylated donkey anti goat IgG	Jackson Immunoresearch –Suffolk, UK 705-005-1447	1:500	1% NHS–0.3% Triton X-100-TBS
Biotinylated goat anti guinea pig	Jackson Immunoresearch –Suffolk, UK 106-005-003	1:500	1% NGS–0.3% Triton X-100-TBS
Biotinylated goat anti rabbit	Vector Labs–Peterborough, UK BA 1000	1:500	1% NGS–0.3% Triton X-100-TBS
Streptavidin Alexa Fluor 594	Molecular Probes –Paisley, UK S11227	1:200	TBS
Streptavidin Alexa Fluor 488	Molecular Probes –Paisley, UK S11223	1:200	TBS
Streptavidin Alexa Fluor 350	Molecular Probes –Paisley, UK S11249	1:50	TBS
Streptavidin Alexa Fluor 635	Molecular Probes –Paisley, UK S32364	1:200	TBS
Streptavidin Alexa Fluor 555	Molecular Probes –Paisley, UK S21381	1:200	TBS