Table 1. Plasmids Constructions and Resulting Polyketide Products.

Each plasmid is a derivative of pRM5. The constructs are transformed into S. coelicolor strain CH999. Produced are analyzed by LC/MS and NMR

^aLM: Loading module, consists of ZhuC, ZhuH, and ZhuG

^bThe minimal PKS consists of the indicated KS-CLF, ZhuN, and the endogenous MAT

^cThis construct contains the act KS-CLF, but lacks the minimal ACP ZhuN

^dNP: no major polyketide product observed

^eNumber in parenthesis indicates yield of the product as percentage of total polyketide recovered