Figure 2.

Neonatal hyperoxia suppresses Ear expression in adult mice. A: Ear protein was detected in lung homogenates of mice exposed to room air (RA) or hyperoxia (O₂) as neonates. Expression of Ear in mice exposed to room air (white bars) or hyperoxia (gray bars) was graphed relative to β-actin (n = 3 to 6 mice per group; *P < 0.05). B: Ear mRNA was detected by semiquantitative RT-PCR. Expression of Ear mRNA in mice exposed to RA or O₂ were graphed relative to β-actin (n = 3 to 6 mice per group; *P < 0.04). C: Lung tissues from mice exposed to room air or hyperoxia at birth were hybridized with antisense or sense Ear1 riboprobe and counterstained with hematoxylin. Thick arrows indicate positive airway staining, and thin arrows depict positive alveolar staining. Scale bar = 100 μm (B). Mean values from a single experiment reproduced three to five times with similar results.