Figure 3.

Electroporation-mediated gene delivery effectively drives high-level expression of Ear1 in the lung. Lungs of adult mice exposed to room air (RA) or hyperoxia (O₂) as neonates were electroporated with empty vector (control) or plasmid-expressing Ear1 (Ear1). A: Lungs were immunostained for Ear (red) and counterstained with DAPI (blue). Scale bar = 100 μmol/L. B: Ear protein was detected by immunoblotting lung homogenates, and levels of expression were graphed as fold change relative to β-actin (n = 3 mice per group; *P < 0.002 and **P < 0.05 compared with RA control and ***P < 0.001 compared with O₂ control).