Figure 6.

Electroporation-mediated gene delivery of Ear1 attenuates fibrotic repair of lungs from infected mice exposed to hyperoxia as neonates. Lungs of adult mice exposed to hyperoxia (O₂) as neonates were electroporated with empty vector control (A, C, and E) or plasmid-expressing Ear1 (B, D, and F). Mice were infected intranasally with influenza A virus 2 days later. Lungs were fixed on postinfection day 14 and stained with H&E (A and B), α-smooth muscle actin (red) with DAPI nuclear counterstain (C and D), or Gomori's trichrome (E and F). Boxes in E and F are magnified below each respective image. Scale bar = 100 μm (A–F).