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. 2012 Aug;181(2):463–471. doi: 10.1016/j.ajpath.2012.04.016

Figure 2.

Figure 2

Knock-down of XIAP exacerbates cell death induced by CSR1. A: Immunoblots of CSR1 and XIAP expression in WCSR1 (PC3 transformed with pCDNA4-CSR1/pCDNA6) cells treated with or without tetracycline, and then treated with siRNA specific for XIAP (siXIAP), scramble control (Scr), or Caspase-9 Inhibitor II (IC, 1 μg/mL). Cells were harvested for analyses 48 hours after transfection. B: Summary of FACS analysis of annexin V and PI staining of WCSR1 cells transfected with a scrambled siRNA (Scr), XIAP-siRNA (siXIAP), or Caspase-9 Inhibitor II (IC), and treated with or without tetracycline. Triplicate experiments of each condition were averaged in the analyses. *P < 0.001. C: Representative images of FACS analyses from B.