Figure 5.
Overexpression of XIAP antagonizes CSR1-induced cell death. A: Immunoblots of CSR1, XIAP, and β-actin expression in WCSR1–2 (PC3 transformed with pCDNA4-CSR1/pCDNA6) cells transfected with pCMVscript (pCMV), pCMV-XIAP, or pCMV-XIAPe314s treated with or without tetracycline. The cell lysates were then separated by SDS-PAGE and identified with the indicated antibodies. B: Summary of FACS analysis of cell death in A. Triplicate experiments of each treatment were analyzed. *P < 0.001. C: Representative images of FACS analysis of annexin V and propidium iodide staining of cells listed in A.