Table 1.
Impact of BCL2 Expression on CSR1-Induced Cell Death
| Cell | Induction | Vector | % Annexin V | % PI | % Annexin V and PI |
|---|---|---|---|---|---|
| WCRS1 | Tet− | pCMVscript | 1.6 ± 0.1 | 8.1 ± 0.4 | 4.7 ± 0.2 |
| Tet+ | pCMVscript | 32.6 ± 0.2 | 2.5 ± 0.3 | 21.3 ± 0.7 | |
| Tet− | pCMV-Bcl2 | 1.9 ± 0.2 | 8.4 ± 0.3 | 5.4 ± 0.4 | |
| Tet+ | pCMV-Bcl2 | 35.8 ± 0.6 | 2.4 ± 0.4 | 21.3 ± 0.8 | |
| WCRS1–2 | Tet− | pCMVscript | 1.8 ± 0.1 | 7.6 ± 0.2 | 4.7 ± 0.1 |
| Tet+ | pCMVscript | 33.4 ± 0.2 | 2.8 ± 0.4 | 20.7 ± 1.3 | |
| Tet− | pCMV-Bcl2 | 2.4 ± 0.3 | 7.5 ± 0.6 | 5.6 ± 0.6 | |
| Tet+ | pCMV-Bcl2 | 30.2 ± 0.7 | 3.0 ± 0.2 | 18.7 ± 0.8 |
pCMV, cytomegalovirus plasmid; Tet, tetracycline; WCSR1, PC3 cells transformed with pCDNA4-CSR1/pCDNA6.