Figure 3.

Development of Th2 immunity to HDM depends on IL-33 and GM-CSF and not TSLP. (A–D) WT and Crfl2^−/− were sensitized and challenged with HDM or PBS as described in Fig. 1. (A) Differential cell counts were determined 72 h later. (B) Levels of serum HDM–specific Igs. (C) Cytokine levels in MLN cells restimulated for 3 d with HDM. (D) TSLP levels measured in BAL fluids. (E–H) WT and Crfl2^−/− were sensitized and challenged with 100 µg HDM or PBS. (E) Differential cell counts were determined 72 h later. (F) Levels of serum HDM–specific Igs. (G) Cytokine levels in MLN cells restimulated for 3 d with HDM. (H) TSLP levels measured in BAL fluids. (I–K) C57BL/6 mice were injected i.p. with blocking soluble ST2 receptor or isotype control at the time of HDM sensitization. (I) Differential cell counts were determined 72 h after the last HDM challenge. (J) Levels of serum HDM–specific Igs. (K) Cytokine levels in MLN cells restimulated for 3 d with HDM. (L–N) C57BL/6 mice were injected i.p. with blocking anti–GM-CSF or isotype control antibodies at the time of HDM sensitization. (L) Differential cell counts were determined 72 h after the last HDM challenge. (M) Levels of serum HDM–specific Igs. (N) Cytokine levels in MLN cells restimulated for 3 d with HDM. *, P < 0.05. Results show one representative experiment out of at least three. Five mice/group were used. Results are shown as mean ± SEM.