Figure 5. Requirement for Lyn in the activity of cathelicidin peptides on mouse macrophages.

BMDM cells from WT and Lyn^−/− mice were stimulated with (A) mouse cathelicidin CRAMP or (B) LL-37 at 50 μg/ml for 24 h and analyzed for CCL4 and CCL7 induction by qRT-PCR. The data were normalized against the GAPDH housekeeping gene and expressed as fold change relative to unstimulated WT sample. Bars represent means ± sem from three animals. *P < 0.05; **P < 0.01 using ANOVA with Bonferroni's multiple comparison post hoc test.