Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Rev Immunol. 2012 Feb 17;12(3):191–200. doi: 10.1038/nri3158

Table 2.

Eight-colour antibody panels proposed by the Human Immunophenotyping Consortium

Fluorochrome Marker
T cells TReg cells TH1, TH2 and
TH17 cells		 B cells DCs, monocytes and
NK cells
FITC Live or dead Live or dead Live or dead Live or dead Live or dead
PE CCR7 CD25 CXCR3 CD24 CD56
PerCP-Cy5.5 CD4 CD4 CD4 CD19 CD123
PE-Cy7 CD45RA CCR4 CCR6 CD27 CD11c
APC CD38 CD127 CD38 CD38 CD16
APC-H7 CD8 CD45RO CD8 CD20 CD3, CD19 and CD20
V450 CD3 CD3 CD3 CD3 CD14
V500 HLA-DR HLA-DR HLA-DR IgD HLA-DR

APC, allophycocyanin; APC-H7, allophycocyanin–cyanine H7 tandem; CCR, CC-chemokine receptor; CXCR3, CXC-chemokine receptor 3; DC, dendritic cell; FITC, fluorescein isothiocyanate; NK, natural killer; PE, phycoerythrin; PE-Cy7, phycoerythrin–cyanine 7 tandem; PerCP-Cy5.5, peridinin chlorophyll protein–cyanine 5.5 tandem; TH, T helper; TReg, regulatory T; V450, violet 450; V500, violet 500.