FIG. 2.

Agarose gel separation of supercoiled (S) and nicked (N) forms of pUC19 plasmid DNA after exposure of plasmid DNA to reactive oxygen species generator reaction. Supercoiled DNA was pre-incubated with water (lanes 2, 4, 5) or increasing concentrations of PrC-210 aminothiol (lanes 3, 6–12) and then exposed to ROS generator (lanes 4–12). Aliquots of each incubation were applied to gel, electrophoresed, stained with EtBr and photographed. Duplicate gels gave the same result.