Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Radiat Res. 2012 Jun 14;178(1):57–68. doi: 10.1667/rr2806.1

FIG. 4.

FIG. 4

Panel A: Growth inhibition of diploid human fibroblasts by the addition of increasing concentrations of PrC-210 aminothiol for the 30 h prior to cell counting. Mean ± SE of multiple wells at each point are plotted. Panel B: Western blot analysis of p21 protein in nuclear extracts from log-phase diploid human fibroblasts that received either control medium or medium containing 4.1 mM PrC-210 (IC70) for the 30 h before cell harvest and isolation of nuclear extracts. Panel C: Flow cytometry analysis of diploid human fibroblasts. Log-phase cells were exposed either to: (1) medium alone (control), (2) medium containing 4.1 mM PrC-210 for the last 30 h before harvest (+PrC-210), or (3) medium containing PrC-210 for 30 h followed by washing (3 medium rinses) and fresh medium for the last 48 h before harvest (+PrC-210 washout). Replicate treatment groups showed the same outcomes.