Table 2.

Effects of FAF-modification on the thermal and thermodynamic stability of NarI-based DNA duplexes^a,^b

entry	−ΔG°c (kcal/mol)	−ΔH°c (kcal/mol)	Tmc,d (°C)	ΔΔG°c,e (kcal/mol)	ΔΔH°c,f (kcal/mol)	ΔTmc,g (°C)
NarI-dC Control	16.2	107.9	68.5
NarI-dC/G3	12.0	65.9	60.2	4.2	42	−8.3
NarI-dC/G1	11.7	76.7	59.1	4.5	31.2	−9.4
NarI-dC/G2	11.6	70.7	61.7	4.6	37.2	−6.8
NarI-dT Control	11.7	77.5	66.3
NarI-dT/G3	11.0	81.9	56.3	0.7	4.4	−10.0
NarI-dT/G1	12.3	85.7	59.3	−0.6	8.2	−7.00
NarI-dT/G2	12.2	121.4	52.6	−0.5	43.9	−13.7

^asee Figure 1 for full structure and sequence details (G* = FAF-adduct).

^bThe results of curve fit and T_m−lnC_t dependence were within ± 15% of each other and therefore these numbers are average of the two methods.

^cThe average standard deviations for −ΔG°, −ΔH°, and Tm are ± 0.22, ± 6.33, and ± 0.4 respectively.

^dT_m values at 14 μM taken from the 1/T_m − lnC_t/4 Meltwin plots

^eΔΔG = ΔG° (FAF-modified duplex) − ΔG° (control duplex).

^fΔΔH = ΔH° (FAF-modified duplex) − ΔH° (control duplex).

^gΔTm = Tm (FAF-modified duplex) − Tm (control duplex).