Table 3.

Effects of FAF-modification on the thermal and thermodynamic stability of NarI/-2 deletion duplexes^a,^b

entry	Sequence	−ΔG° c (kcal/mol)	−ΔH°c (kcal/mol)	Tmc,d (°C)	ΔΔG°c,e (kcal/mol)	ΔΔH°c,f (kcal/mol)	ΔTmc,g (°C)
NarI-dC/-2 Control	-GGCG*CC- -CC-- GG-	8.2	30.5	54.5
NarI-dC/G3*/-2 del	-GGCG*CC- -CC-- GG-	11.2	91.3	57.8	−3.0	−60.8	3.3
NarI-dT/-2 Control	-GGCG*CT- -CC-- GA-	8.5	30.5	45.7
NarI-dT/G3*/-2 del	-GGCG*CT- -CC-- GA-	9.0	58.5	44.7	+0.5	−28.0	−1.0

^asee Figure 1 for full structure and sequence details (G* = FAF-adduct).

^bThe results of curve fit and T_m−lnC_t dependence were within ± 15% of each other and therefore these numbers are average of the two methods.

^cThe average standard deviations for −Δ G°, −ΔH°, and Tm are ± 0.22, ± 6.33, and ± 0.4 respectively.

^dT_m values at 14 μM taken from the 1/T_m − lnC_t/4 Meltwin plots

^eΔΔG = ΔG° (FAF-modified duplex) − ΔG° (control duplex).

^fΔΔH = ΔH° (FAF-modified duplex) − ΔH° (control duplex).

^gΔTm = Tm (FAF-modified duplex) − Tm (control duplex).