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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Cancer Res. 2009 Sep 8;69(18):7243–7251. doi: 10.1158/0008-5472.CAN-09-0167

Figure 1.

Figure 1

Relative CSC content in C6 Ser+ and SFS cultures and tumors. A, tumor sphere-forming assay: 100 cells plated per well. B, cytospins of C6 cultures stained for nestin (Texas red; red). Nuclei stained with 4′,6-diamidino-2-phenylindole (blue), ×200. Histogram: image analysis of nestin staining. Eight fields analyzed per group. Cells displaying Texas red fluorescence above background (established using Texas red–conjugated IgG control) were counted as nestin-positive. % Nestin-positive cells per field calculated as nestin-positive cells/DAPI–stained cells (counted manually). C, ex-vivo tumor sphere-forming assay using established tumors (4 wk post-implantation).

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