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Microvessel density and blood perfusion in CSC-low and CSC-high tumors. A, top, CD31+ microvessels (Cy3; red), ×200; bottom, blood perfusion (Hoechst; blue), ×100. B, image quantification of microvessel density: number of CD31+ (red) structures per field (counted manually). C, image quantification of blood perfusion: total Hoechst fluorescence intensity (total colored pixels × mean fluorescence intensity) per field. Five mice per group, five fields analyzed per tumor.
