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VEGF- and SDF1-blocking experiments in mice with CSC-low and CSC-high tumors. A and B, image quantification of microvessel density in tumors ± DC101 (anti-VEGFR2) or AMD3100: number of CD31+ structures per field (counted manually). Five mice per group, eight fields per tumor. C, quantification of vessel-associated, bone marrow–derived (GFP+) endothelial cells (CD31+/VEGFR2+/CD45−) ± AMD3100 per 10,000 tumor cells by flow cytometry. Five mice per group, except CSC-low control (n = 3).
