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Limiting dilution tumor initiation assay
| No. C6 cells injected | Tumor take (sites engrafted/injected)
|
|
|---|---|---|
| Injecting Ser+ | Injecting SFS | |
| 50,000 | 10/10 | 10/10 |
| 10,000 | 10/10 | 10/10 |
| 5,000 | 3/10 | 9/10 |
| 1,000 | 0/10 | 0/10 |
| 100 | 0/10 | 0/10 |
NOTE: Five mice per dilution, right and left flanks were injected, for a total of 10 injections per dilution. Results at 5,000 cell dilution are significantly different (P = 0.0198, Fisher’s exact test).
