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. 2012 Jul 20;3(8):1898–1913. doi: 10.1364/BOE.3.001898

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© 2012 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 5 — Aberration measurement on fixed Brainbow mouse hippocampus mounted in Vec-tashield. (a) and Media 6^{(9.9MB, AVI)}, CFP and endogenous 2PEF image at depths of 10, 100 and 200μm. (b)–(d), aberration coefficient maps for (b), astigmatism (z=5); (c), coma (z=8); (d), spherical aberration (z=11). The three maps share the same colour scale. (e), maps of the total aberration amplitude. Scale bar, 100μm. At each depth, measurement of the 11 aberration maps took about 6 minutes and induced ≈ 7% decrease of the fluorescent signal due to photobleaching.