Fig. 6.

Deep imaging within fixed mouse hippocampus. (a) and Media 7 ^{(46.7MB, AVI)} , comparison of 2PEF imaging over a 400 × 400 × 420μm volume of a PBS-mounted (left and a Vectashield-mounted (right) sample. The lines on the images indicate the location of the three visualised planes. Top, xy view (cut along the orange lines); middle, xz view (cut along the yellow lines); bottom, yz view (cut along the blue lines). Scale bar, 100μm. (b), residual aberration correction on a Vectashield-mounted sample. Within a 100 × 100μm aplanetism region, the quality of images can be maintained throughout the tissue by adjusting the correction phase. Scale bar, 50μm.