Table II.
Specific activity of various GT-42 family members on synthetic acceptors
| Substratea | Structure | CstI | CstII | HBS-02 | HAC1267 | HAC1268 | PM1174 |
|---|---|---|---|---|---|---|---|
| Lactose | Gal-β1,4-Glc | 785.8b | 455.5 | <0.1 | 2.7 | 6.1 | 58.9 |
| LacNAc | Gal-β1,4-GlcNAc | 715.7 | 136.5 | 14.9 | 28.1 | 58.2 | 56.1 |
| LNB | Gal-β1,3-GlcNAc | 924.2 | 733.1 | 1.4 | 7.1 | 0.67 | 8.9 |
| T-Antigen | Gal-β1,3-GalNAc-α | 401.7 | 57.3 | 0.34 | 0.19 | <0.1 | 0.47 |
| GM3 | NeuAc-α2,3-Gal-β1,4-Glc | 1.96 | 1002 | 0 | 0 | 0 | 0 degrades |
aAll substrates were made as aminophenyl-glycosides that were labeled with fluorescein (FCHASE or FEX) as described in Wakarchuk and Cunningham (2003), and enzymatically converted to the substrates as previously described (Blixt et al. 2005; Bernatchez et al. 2007; Namdjou et al. 2008)
bmU/mg of protein using a standard assay condition. Glycoside linkages are bolded to emphasize the acceptor specificity.