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. 2012 Jul 9;109(30):12171–12176. doi: 10.1073/pnas.1201660109

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Fig. 5. — SDs in R1a increase the surface stability of R1aR2. (A) Hippocampal neurons expressing either R1a^BBSΔSD1R2 or R1a^BBSΔSD2R2 with eGFP were incubated in 1 mM d-TC, followed by 3 μg/mL BTX-AF555, for 10 min at RT and imaged at different time points at 30–32 °C. (B) Rates of internalization for BTX-AF555 tagged R1a^BBSR2 (●), R1b^BBSR2 (□), R1a^BBSΔSD1R2 (), and R1a^BBSΔSD2R2 () receptors (n = 7–12). (C and D) Exponential decay time constants for membrane fluorescence (C) and extent of constitutive internalization (D) of R1a^BBSR2, R1b^BBSR2, R1a^BBSΔSD1R2, and R1a^BBSΔSD2R2. ***P < 0.001; **P < 0.01, *P < 0.05 (one-way ANOVA). (Scale bar: 10 μm.)

Inline graphic — SDs in R1a increase the surface stability of R1aR2. (A) Hippocampal neurons expressing either R1a^BBSΔSD1R2 or R1a^BBSΔSD2R2 with eGFP were incubated in 1 mM d-TC, followed by 3 μg/mL BTX-AF555, for 10 min at RT and imaged at different time points at 30–32 °C. (B) Rates of internalization for BTX-AF555 tagged R1a^BBSR2 (●), R1b^BBSR2 (□), R1a^BBSΔSD1R2 (), and R1a^BBSΔSD2R2 () receptors (n = 7–12). (C and D) Exponential decay time constants for membrane fluorescence (C) and extent of constitutive internalization (D) of R1a^BBSR2, R1b^BBSR2, R1a^BBSΔSD1R2, and R1a^BBSΔSD2R2. ***P < 0.001; **P < 0.01, *P < 0.05 (one-way ANOVA). (Scale bar: 10 μm.)