Fig. 6.

SDs increase surface stability of R1aR2 in dendrites and spines. (A and B) Hippocampal neurons expressing either R1a^BBSR2 or R1b^BBSR2 with eGFP were incubated in 1 mM d-TC, followed by 3 μg/mL BTX-AF555, for 10 min at RT and spines (A) or dendrites (B) imaged up to 60 min at 30–32 °C. (C and D) Rates of constitutive internalization of R1a^BBSR2 (●) and R1b^BBSR2 (□) on dendritic spines (n = 75–78) (C) and membranes (n = 7–10) (D). (E) Exponential decay time constants for membrane fluorescence of R1a^BBSR2 and R1b^BBSR2 in dendrites (Left) and spines (Right). (F) Extent of constitutive internalization of R1a^BBSR2 and R1b^BBSR2 in dendrites (Left) and spines (Right). NS, not significant; ***P < 0.001; **P < 0.01. (Scale bar: 2 μm.)