Fig. 7.

SDs stabilize cell surface mGluR2 receptors. (A) Schematic diagram showing the location of the BBS in mGluR2. The BBS was inserted between Lys6 and Val7. (B) Hippocampal neurons expressing either mGluR2^BBS or mGluR2^BBS-SD were incubated in 1 mM d-TC, followed by 3 μg/mL BTX-AF555, for 10 min at RT and imaged up to 60 min at 30–32 °C. (C) Rates of constitutive internalization for BTX-AF555–tagged mGluR2^BBS (▽ ), mGluR2^BBS-SD (▲), R1a^BBSR2 (●), and R1b^BBSR2 (□) (n = 7–12). (D and E) Exponential decay time constants for membrane fluorescence (D) and extents of constitutive internalization (E) of mGluR2^BBS, mGluR2^BBS-SD, R1a^BBSR2, and R1b^BBSR2. ***P < 0.001; **P < 0.01; *P < 0.05 (one-way ANOVA). (Scale bar: 10 μm.)