Fig. 1.

Hematopoietic-specific miR-142 inhibits replication of targeted IAV infection. (A) MDCK and MDCK142 cells infected with NPctrl and NP142t IAV at a multiplicity of infection (MOI) of 2 and 16 hours post infection (hpi) analyzed for svRNA (Upper) and U6 (Lower) expression. (B) Cells infected as in A at a MOI of 0.1 and 16 hpi analyzed for NP (Upper) and actin (Lower) protein expression. (C) MDCK and MDCK142 cells infected with NPctrl or NP142t IAV at an MOI of 0.001 and virus titered from supernatants at the indicated time points. (D) BMM infected with NPctrl or NP142t IAV at an MOI of 5 and protein analyzed as in B. (E) Primary lung fibroblasts infected with NPctrl or NP142t IAV at an MOI of 2 and protein analyzed as in B. Data are representative of two to three independent experiments.