Fig. 2.

Inhibition of IAV replication in hematopoietic cells in vivo does not alter disease progression or virus titers. (A) Mice were infected intranasally with 10⁵ pfu of NPctrl or NP142t IAV and 2 dpi CD45⁺CD11c⁺ (Left) and CD45⁻ cells (Right) from the lungs were analyzed for surface HA expression by FACS; P = 0.0258 and P = 0.3284 for CD45⁺CD11c⁺ and CD45⁻ cells, respectively. (B) Mice were infected as in A and titered from lung draining LN (Left) and lungs (Right) 2 dpi. P = 0.0107 and P = 0.282 for LN and lungs, respectively. (C) Mice were infected with NPctrl or NP142t IAV and monitored daily for weight loss. (D) Mice were infected as in C and lungs were removed at indicated time points and virus titers were assessed by plaque assay. Samples not detected were arbitrarily plotted below the limit of detection (LOD). All Data are representative of two independent experiments with three to five mice per group.