Fig. 3.

The Sin3a complex negatively regulates the transcription of a subset of STAT3-dependent genes. (A) Venn diagram showing the number of LIF-induced genes in cell transfected with control siRNA (Renilla luciferase, yellow), with SIN3A siRNA (blue), or in both the conditions (green). As cutoff, a log₂-ratio > 1 (more than two times up-regulated) was applied to analyze the gene expression profile. (B) MCF7 cells were transfected with control (Renilla luciferase) or SIN3A siRNA. After 72 h, cells were left nonstimulated or stimulated with LIF. Graphs represent mRNA levels relative to NS sample. Results are representative of three independent experiments. Error bars indicate SD from triplicates. ***P < 0.001; one-way ANOVA with Bonferroni test.