Fig. 1.

Glycine receptor subunits undergo developmental expression changes over the first three postnatal weeks. L4 spinal sections were stained for both the α₁/β heteromeric receptor (Upper) and the α₂ subunit (Lower) of the glycine receptor. (Insets) Immunopositive cells used for analysis. (B) Ratio of α₂⁺:α₁/β⁺ neurons. At P3, expression of α₂ is greater than α₁/β in the dorsal horn, but the ratio reverses at P14 and P21. Data presented as mean ± SEM, *P < 0.05, **P < 0.01. n = 4 animals per group, five sections per animal. Identical section appears in Upper and Lower panels at each age.