Fig. 1.

Identification of a unique TSS in IL-1R1 gene. (A) Diagrammatic illustration of the half-nested PCR used to increase the sensitivity of 5′ RACE. The forward RACE primers were included in the CapFishing kit. The reverse primers for two rounds of PCR targeting different exons are indicated by arrows (purple, outer primers for first round of PCR; green, inner primers for second round of PCR). (B) Electrophoresis results of the nested PCR results targeting TSS downstream of exon 2. RNAs used in CapFishing were extracted from the following tissues: dentate gyrus (Dg), hypothalamus (Hy), liver (Liv), and lung (Lun). (C) Annotation of TSSs found in the context of known mIL-1R1 genomic sequence. TSSs are presented in green, purple, blue, and red to match Table 1.