Skip to main content
. 2012 Jul 9;109(30):12189–12194. doi: 10.1073/pnas.1205207109

Fig. 4.

Fig. 4.

Role of IL-1R3 in mediating IL-1β–induced signaling. (A) IL-1β induces NF-κB activation. Fold increase in luciferase activity over the baseline obtained from cells cotransfected with NF-κB reporter plasmid and empty vector (V) are presented. The means ± SE are presented from three separate experiments. *P < 0.05. (B) Detection of IL-1β–induced phosphorylation of Akt and p38 in cells transfected with IL-1R1/IL-1R3 in combination with IL-1RAcP/IL-1RAcPb. *, indicates increased phosphor-protein. (C) Detection of IL-1β–induced phosphorylation of Akt in cells transfected with IL-1R3 in combination with IL-1RAcP/IL-1RAcPb. Quantification of blots from three different experiments is shown below the blot. Densitometric values are expressed as pAkt/total Akt and then normalized to untreated cells (0 min). *P < 0.05. (D) Time course of IL-1β–induced Akt phosphorylation in cells cotransfected with IL-1R3 and IL-1RAcPb.