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Typical absorption spectra of hemoglobin (1 × 10−5M, solid line, free) and with 6 × 10−6 M (- - -); 1.5 × 10−5 M (-.-.) of diadzein (see left axis). Right axis presents the absorption of 1 × 10−5 M free diadzein in aqueous phase (-..-..). Inset: Fluorescence spectra of 1.0 × 10−5 M diadzein in aqueous phase (-..-..) and protein matrix (-----). λex=340 nm.
