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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Int J Biol Macromol. 2012 May 16;51(3):250–258. doi: 10.1016/j.ijbiomac.2012.05.013

Figure 4.

Figure 4

Variation in fluorescence anisotropy (r) of diadzein (1.0 × 10−5 M) with increasing protein (HbA) concentration (λex = 340 nm; λem = 470 nm, excitation and emission bandwidths were 5 nm). Each data point indicates the average of three determinations. Inset: Circular dichroism spectra of 3.0 × 10−6 M hemoglobin (HbA) in absence (solid line) and presence of 10.0 × 10−6 M diadzein (dotted line) in 0.01 M phosphate buffer, pH 7.4.