Table 1. Effects of confined space on growth rate in Astyanax mexicanus surface fish (SF), Pachón cavefish (CF), Tinaja cavefish (CF), and F1 and F2 hybrids.

					Mean Growth (+/− SE)		ANOVA (p)		Post Hoc Testa (p)
Subject	Conditions	Duration (days)	N	Volume (ml)	Mass (mg/d)	Length (mm/d)	Mass	Length	Mass	Length
SF	Static, Light	32	12	10	1.19+/−0.05	0.11+/−0.01	0.000	0.002	<0.01	<0.01
SF	Static, Light	32	12	40	1.97+/−0.07	0.15+/−0.01
SF	Flow, Light	27	6	10	0.36+/−0.15	0.03+/−0.02	0.006	0.000	<0.01	>0.05
SF	Flow, Light	27	6	40	1.00+/−0.11	0.09+/−0.01
CF Pachón	Static, Light	32	11	10	2.39+/−0.09	0.22+/−0.01	0.000	0.576	<0.01	–
CF Pachón	Static, Light	32	11	40	2.98+/−0.10	0.21+/−0.01
CF Pachón	Flow, Light	27	5	10	0.21+/−0.10	0.04+/−0.01	0.186	0.705	–	–
CF Pachón	Flow, Light	27	6	40	0.53+/−0.18	0.03+/−0.02
CF Tinaja	Static, Light	11	9	10	1.28+/−0.19	0.10+/−0.01	0.817	0.360	–	–
CF Tinaja	Static, Light	11	6	40	1.34+/−0.13	0.12+/−0.02
CF Tinaja	Flow, Light	27	4	10	0.46+/−0.24	0.06+/−0.02	1.000	1.000	–	–
CF Tinaja	Flow, Light	27	4	40	0.46+/−0.13	0.06+/−0.01
SF	Static, Dark	36	10	10	1.39+/−0.24	0.06+/−0.01	0.000	0.000	<0.01	<0.01
SF	Static, Dark	36	13	40	2.59+/−0.16	0.11+/−0.01
F1 Hybrid	Static, Light	18	10	10	1.67+/−0.15	0.15+/−0.01	0.000	0.000	<0.01	<0.01
F1 Hybrid	Static, Light	18	9	40	4.44+/−0.11	0.29+/−0.02
F2 Hybrid	Static, Light	32	10	10	2.19+/−0.11	0.19+/−0.02	0.005	0.260	<0.01	–
F2 Hybrid	Static, Light	32	12	40	2.66+/−0.09	0.21+/−0.01

^aGames-Howell post-hoc tests were used to analyze the data when Levene’s test was significant (p<0.05) and Tukey’s post hoc tests were used when Levene’s test was not significant (p>0.05).