Table 1. Pseudomonas syringae pv. maculicola ES4326 transposon disruptants with reduced virulence in a high-throughput liquid media-based assay.
| Groupa | Gene Affected | Number Identified | (Putative) Protein Function | Location Within Operon |
| I | hrcN | 5 | ATPase | hrpJ-hrcV-hrpQ- hrcN -hrpO-hrpP-hrcQa-hrcQb-hrcR-hrcS-hrcT-hrcU |
| hrcQb | 2 | type III secretion-associated protein | hrpJ-hrcV-hrpQ-hrcN-hrpO-hrpP-hrcQa- hrcQb -hrcR-hrcS-hrcT-hrcU | |
| hrpS | 1 | TTSS transcriptional regulator | hrpR- hrpS | |
| hrpG | 1 | TTSS regulatory protein | hrpF- hrpG -hrcC-hrpT-hrpV | |
| hrpQ | 1 | inner membrane structural TTSS component | hrpJ-hrcV- hrpQ -hrcN-hrpO-hrpP-hrcQa-hrcQb-hrcR-hrcS-hrcT-hrcU | |
| hrpB | 1 | ATP-dependent helicase | hrpZ1- hrpB -hrcJ-hrpD | |
| hrcJ | 2 | lipoprotein; inter-membrane bridge | hrpZ1-hrpB- hrcJ -hrpD | |
| hrcU | 1 | TTSS inner membrane component | hrpJ-hrcV-hrpQ-hrcN-hrpO-hrpP-hrcQa-hrcQb-hrcR-hrcS-hrcT- hrcU | |
| hrpO | 1 | regulator of TTSS protein export | hrpJ-hrcV-hrpQ-hrcN- hrpO -hrpP-hrcQa-hrcQb-hrcR-hrcS-hrcT-hrcU | |
| hrcS | 1 | TTSS structural component | hrpJ-hrcV-hrpQ-hrcN-hrpO-hrpP-hrcQa-hrcQb-hrcR- hrcS -hrcT-hrcU | |
| hrcV | 1 | TTSS structural component | hrpJ- hrcV -hrpQ-hrcN-hrpO-hrpP-hrcQa-hrcQb-hrcR-hrcS-hrcT-hrcU | |
| hrpR | 1 | TTSS transcriptional regulator | hrpR -hrpS | |
| hrcC | 1 | outer membrane TTSS protein | hrpF-hrpG- hrcC -hrpT-hrpV | |
| II | fleN | 1 | negative regulator of flagellar number | fleN -fliA |
| fliH | 2 | putative flagellar assembly protein | fliE-fliF-fliG- fliH -fliI-fliJ | |
| flgF | 1 | flagellar basal-body rod protein | flgF -flgG-flgH-flgI-flgJ-flgK-flgL | |
| fliG | 1 | flagellar motor switch protein | fliE-fliF- fliG -fliH-fliI-fliJ | |
| flgH | 1 | flagellar L-ring protein | flgF-flgG- flgH -flgI-flgJ-flgK-flgL | |
| III | galU | 1 | UTP-glucose-1-phosphate uridylyltransferase | single gene |
| mdoG | 1 | periplasmic glucan biosynthesis | mdoG -mdoH | |
| mdoH | 1 | periplasmic glucan biosynthesis | mdoG- mdoH | |
| IV | gltB | 3 | glutamate synthase (large subunit) | single gene |
| hisD | 2 | histidinol dehydrogenase - histidine biosynthesis | hisD -hisC | |
| serB | 1 | phosphoserine phosphatase - serine biosynthesis | single gene | |
| V | cynT | 3 | carbonic anhydrase | single gene |
| cbrA | 1 | sensory box histidine kinase | cbrA -cbrB | |
| kinB | 1 | sensory box histidine kinase | algB- kinB |
a
Disruptants are grouped according to the known or predicted functions of the disrupted genes as follows: type III secretion (Group I), flagellar motility (Group II), periplasmic glucan biosynthesis (Group III), amino acid biosynthesis (Group IV), or other metabolic processes (Group IV).