Table 4. Mapped barley miRNAs in miRBase.
miRNA | miRNA sequence | GPread count | GP(TaDREB3)read count | GP(TaDREB3)normalised | log2 |
hvu-miR5048 | UAUUUGCAGGUUUUAGGUCUAA | 4547 | 2106 | 2912.955844 | −0.642431171 |
hvu-miR159a/bhvu-miR159a* | UUUGGAUUGAAGGGAGCUCUGGAGCTCCTATCATTCCAATGA | 22465 | 42602 | 5892.303845 | 1.391473899 |
hvu-miR444b | UGCAGUUGCUGUCUCAAGCUU | 64 | 30 | 41.4950975 | −0.625131008 |
hvu-miR1120 | ACAUUCUUAUAUUAUGGGACGGAG | 387 | 94 | 130.0179722 | −1.573622508 |
hvu-miR444a | UUGCUGCCUCAAGCUUGCUGC | 1 | 0 | 0 | #NUM! |
hvu-miR156 | UGACAGAAGAGAGUGAGCACA | 140769 | 213440 | 295223.787 | 1.068479308 |
hvu-miR1436 | ACAUUAUGGGACGGAGGGAGU | 8 | 2 | 2.766339833 | −1.532021604 |
hvu-miR5050 | UUGAGGUCGUUCAACCAGCAA | 57 | 27 | 37.34558775 | −0.610024116 |
hvu-miR5051hvu-miR5051* | UUUGGCACCUUGAAACUGGGACCAGTTTCAAGGTTTCAAAGC | 41 | 50 | 6.915849583 | 0.789906491 |
hvu-miR171 | UGAUUGAGCCGUGCCAAUAUC | 158 | 88 | 121.7189527 | −0.376370733 |
hvu-miR168-3phvu-miR168-3p* | GAUCCCGCCUUGCACCAAGUGAAUCCCGCCTTGCACCAAGTGAAT | 404324 | 293248 | 405.2687856 | 0.004523768 |
hvu-miR399 | UGCCAAAGGAGAUUUGCCCCG | 2 | 1 | 1.383169917 | −0.532021604 |
hvu-miR5049 | UCCUAAAUACUUGUUGUUGGG | 549 | 121 | 167.3635599 | −1.713820705 |
hvu-miR397 | CCGUUGAGUGCAGCGUUGAUG | 8 | 7 | 9.682189416 | 0.275333318 |
hvu-miR166/b/c | UCGGACCAGGCUUCAUUCCCC | 19883 | 6967 | 9636.544809 | −1.044947586 |
hvu-miR1126 | UCAACUAUGGACUACAUACGGAA | 13 | 0 | 0 | #NUM! |
hvu-miR5052 | ACCGGCUGGACGGUAGGCAUA | 11 | 3 | 4.14950975 | −1.406490722 |
hvu-miR168-5p | UCGCUUGGUGCAGAUCGGGAC | 420777 | 179792 | 248682.8857 | −0.758748623 |
hvu-miR5053 | CGCAGCUGUAGUCGCCGGCGU | 24 | 5 | 6.915849583 | −1.795056009 |
Note: GP-Golden Promise barley;
GP(TaDREB3)-Golden Promise transgenic barley over-expressing TaDREB3;
miRNA*, star sequence and read count are highlighted in blue.