Table 9. Detected target sequences of novel miRNAs in barley degradome library.
| miRNA | miRNA sequence | Target cleavage site | Target accession (0 mismatches) | Annotation |
| Hvu-miRX1 | GAATGACGCCGGGTCCGAAAG | 10/11: AGGCGTCATTCAAATTTCTG | Not available | Not available |
| Hvu-miRX2 | AGTGACGCGCATGAATGGATT | 10/11: GCGCGTCACTGCAACGGATAA10/11: GCGCGTCACTAATTAGATGAC12/13: ATGCGCGTCACTAATTAGATG13/14:CATGCGCGTCACTAATTAGAT | Not available for “GCGCGTCACTGCAACGGATAA”BY864760/BY865136/BY859056/BY865253/BY861299/BY839718/TC275169/BY864507/TC281249/AL508349/BI951089/TC279957/AJ432484/AU252321/TC281472/BI949016/TC281181/TC281158/TC257287/TC257303/BI953026/TC262239/TC278355/TC261623/TC268219/TC275184/TC274467/TC273938/TC268772 | P450-like TBP,prolin rich protein,B hordein precursor,6-phosphogluconate dehydrogenase,decarboxylating,ribosome protein |
| Hvu-miRX3 | TGCTCACTGCTCTATCTGTCACC | 10/11: GCAGTGAGCAGCCGGGTTAA | Not available | Not available |
| Hvu-miRX4 | AGGTGTCATCCCGCCTGAACA | 10/11: GATGACACCTTTAGAGCGGG | dbj|AK356860.1| | unknown protein |
| Hvu-miRX5 | ACTGGTTGGATCATGCTTCTC | 10/11: TCCAACCAGTCAGATCCAACCA10/11: TCCAACCAGTCCGACGATCCAA11/12: ATCCAACCAGTCCGACGATCCA12/13: GATCCAACCAGTCCGACGATCC | Not available for all sequences | Not available |
| Hvu-miRX6 | ATTTACTTGTAGAAGAAGCTA | 10/11: ACAAGTAAATACGACATGGG | gb|HQ825319.1|/gb|AF147501.1| | ribosome protein |
| Hvu-miRX7 | TCAGATGAGAAGGCAGATCATA | 10/11: TCTCATCTGAGTTTGATCCT10/11: TCTCATCTGAAATATGATCCT | Not available for all sequences | Not available |
| Hvu-miRX8 | TAGGAAAGTAGAGTAGGCACA | 11/12: CTACTTTCCTACAAAGTTAT | Not available | Not available |
| Hvu-miRX9 | ATTGACGACCTAGATACACGTGCA | 12/13: TAGGTCGTCAATCCTACCAT | Not available | Not available |
| Hvu-miRX10 | TCTGTAACTTAATATAAGACG | 14/15: TATTAAGTTACAGAGGGAGAG | Not available | Not available |
| Hvu-miRX11 | TATTTGCAGGTTTTAGGTCTAA | 10/11: CCTGCAAATAATATAGCATGT10/11: CCTGCAAATACCTGGTTCTC | Not available for all sequences | Not available |
| Hvu-miRX12 | AATTATTTAGGTACAGAGGGA | 10/11: CTAAATAATTTAAAAATGGA | Not available | Not available |
| Hvu-miRX13 | AATTAATATGGATCGGAGGGA | 10/11: CATATTAATTTCGACAACCC | Not available | Not available |
| Hvu-miRX14 | CCGAACTGATGGAAAGGGCTA | 10/11: ATCAGTTCGGCAGAGAGCAGA | Not available | Not available |
| Hvu-miRX15 | TTTTGGTTGCGTTGGCTAGTGCAT | 10/11: GCAACCAAAAGTCCGAACTAA | CA023038 | DNA-directed RNA polymerase subunit |
| Hvu-miRX16 | TATAGTAATGATGGCTAATGGT | 10/11: CATTACTATACCTTCCCTATT | gb|EF115541.1|/emb|X14108.1|/gb|M35616.1|/BLYCPPSBEF/gb|M35977.1|/BLYCPBEF/TC276030 | psbE and psbF proteins,b559 apoprotein |
| Hvu-miRX17 | TTAGGATTAGGAATAGGTGTA | 10/11: CTAATCCTAAGGATTCCTTAA | Not available | Not available |
| Hvu-miRX18 | AAATAGAATAATGATCAACGGA | 10/11: TATTCTATTTCACTATTTCA10/11: TATTCTATTTTTGTTGGATC | BE437950/gb|EF115541.1|Not available for “TATTCTATTTTTGTTGGATC” | unknown protein |
| Hvu-miRX19 | TTTGAGGGTTCTAGTCTTTGC | 10/11: GTGTAGAACCCTCAAATGAG | Not available | Not available |
| Hvu-miRX20 | ATCGTATGAACTTGAAGCAACGGT | 10/11: TTCATACGATCCAACGAGTTC | Not available | Not available |
Note: Sequences highlighted in red in each row are from the same target. Target cleavage sites are indicated by numbers relative to the guiding miRNAs from 5′ to 3′.