Table 2.
Reduced density of VGLUT1- and GAD65-mRNA expressing neurons in the subiculum and layer III of the EC. Densities of neurons (number of neurons/mm2) were quantified in darkfield photomicrographs of photoemulsion-dipped sections after in situ hybridization and expressed as % of controls ± SEM. Data for early intervals (24 h and 8 days) and late intervals after KA-induced SE (1 and 3 months) were pooled, respectively. Below the densities of VGLUT and GAD65-mRNA expressing neurons in the respective controls are shown (ANOVA with Dunnett’s multiple post hoc test; *P < 0.05; **P < 0.01; ***P < 0.001).
| n | Proximal Sub | Distal Sub | Medial EC III | Lateral EC III | |
|---|---|---|---|---|---|
| VGLUT1 mRNA | |||||
| KA, 1–8 days (% of control) | 11 | 56 ± 6.9%∗∗∗ | 68 ± 6.4%∗∗∗ | 39 ± 8.2%∗∗∗ | 76 ± 2.7%∗∗∗ |
| KA, 1–3 months (% of control) | 18 | 58 ± 4.2%∗∗∗ | 74 ± 4.0%∗∗∗ | 38 ± 6.3%∗∗∗ | 72 ± 3.2%∗∗∗ |
| Controls (neurons/mm2) | 13 | 848 ± 32.2 | 894 ± 27.2 | 861 ± 34.3 | 779 ± 27.7 |
| GAD65 mRNA | |||||
| KA, 1–8 days (% of control) | 11 | 64 ± 6.4%∗∗∗ | 79 ± 3.1%∗∗ | 73 ± 4.0%∗∗ | 73 ± 7.5%∗ |
| KA, 1–3 months (% of control) | 18 | 67 ± 3.8%∗∗∗ | 78 ± 4.5%∗∗ | 68 ± 3.9%∗∗∗ | 86 ± 3.7% |
| Controls (neurons/mm2) | 13 | 114 ± 6.2 | 102 ± 5.9 | 82 ± 7.6 | 68 ± 7.4 |