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. Author manuscript; available in PMC: 2012 Aug 2.
Published in final edited form as: Nature. 2011 Oct 23;479(7374):552–555. doi: 10.1038/nature10545

Figure 2.

Figure 2

Confocal microscopy of syntaxin-1A domains in artificial membranes. Syntaxin-1A257–288 labeled with Atto647N (SxTMH; red) was reconstituted in GUVs. The membranes were composed of DOPC with 1.5 mol% of the fluorescent lipid analog DiO (3,3’-dioctadecyloxacarbocyanine; green) and the percentages DOPS, cholesterol and PIP2 indicated in the figure. In absence of anionic phospholipids, 20% cholesterol clustered syntaxin-1A in many small clusters (condition #2), as predicted by Murray and Tamm17. Inclusion of > 5% anionic DOPS dispersed these clusters (#3). 1.5% PIP2 partitioned SxTMH in 1–10 μm-sized domains regardless of cholesterol or DOPS (#4–8). These clusters were no longer observed with 5% PIP2 (#9). The pink arrows show the part of the membrane used for cross-sections. Yellow bars indicate the position of the domains. More data is presented in Supp. Fig. 4–9.