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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Leukemia. 2012 Apr 30;26(11):2375–2383. doi: 10.1038/leu.2012.117

Figure 4. Effect of neutrophil on osteolineage cells survival.

Figure 4

FACS sorted Ly6Gpos neutrophil and Ly6Gneg non neutrophil BM cells were co-cultured either with MC3T3 pre-osteoblast cells or differentiated OB in presence of G-CSF for 3 days. (A) MC3T3 cells apoptosis and (B) differentiated OB apoptosis was determined by flow cytometry after Annexin V staining (X ± SEM; N=3 experiment). (C) Annexin V expression on BM CD45− CD31− Ter119− osteolineage cell from the G-CSF treated control or neutropenic mice (X ± SEM; N=5 mice in each group). *P< 0.05 compared to vehicle; † P< 0.05 compared to isotype plus G-CSF; ¶ P > 0.05 compared to vehicle.