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. 2012 Jun;135(6):887–894.

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Copyright: © The Indian Journal of Medical Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Histological examination of the corneas after the surgery. (A) HE-stained cross-section showed that Descemet's membrane was covered by cell monolayer in the B4G12 cell-injection group. (B) No cells were present on Descemet's membrane in the cryoinjury only group. Note that the corneal stroma was much thicker than that in the B4G12 cell-injection group. (C) Fluorescence microscopy confirmed that cells covering the Descemet's membrane in the B4G12 cell-injection group were CFDA SE-positive. (D) No signal was detected from the corneas of the cryoinjury only group. Scale bars: 100 μm.