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. 2012 Jun;135(6):887–894.

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Copyright: © The Indian Journal of Medical Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — Immunofluorescent determination for ZO-1 and Na+/K+ ATPase. (A and a) ZO-1 was mainly expressed at the cell borders of corneal endothelium equivalents. (B and b) Na⁺/K⁺ ATPase was detected in the cell plasma and membrane of corneal endothelium equivalents. (C and c) Negative control in which the primary antibody was omitted for ZO-1. (D and d) Negative control in which the primary antibody was omitted for Na⁺/K⁺ ATPase. A, B, C and D are plane views of corneal endothelium equivalents and a, b, c and d are corresponding cross-section views. Scale bars: 50 μm.