Outline of visit 3. Bedside glucose was measured every 10 min for adjustment of the GIR. Blood was collected every 30 min for measurement of glucose, insulin, and FFA. Three muscle biopsies were obtained from the vastus lateralis (biopsy 1, time 0 min; biopsy 2, time 120 min; biopsy 3, time 360 min) over the course of the lipid/glycerol infusion.