Table 1.
Lineage-specific mutant and transgenic lines for zebrafish hematopoiesis research.
Lineage | Marker | Mutant lines | Transgenic lines | ||
---|---|---|---|---|---|
Mutant designation and mutation type | References | Line designation | References | ||
Hemangioblast | tal1/scl | t21384, K183X | [38] | PAC-tal1:GFP 5.0tal1:EGFP | [39, 40] |
lmo2 | None | lmo2:EGFP lmo2:DsRed | [41] | ||
| |||||
EMPs | runx1 | hg1, W84X | [42, 43] | runx1P1:EGFP | [44] |
| |||||
runx1 | hg1, W84X | [42, 43] | runx1P2:EGFP | [44] | |
HSCs | cmyb | t25217, I181N hkz3, truncation in transactivation domain |
[45] [46] |
cmyb:EGFP | Developed by the Zon lab, used in [47] |
cd41 | None | cd41:GFP | [33, 34] | ||
| |||||
Erythropoiesis | gata1 | m651 (vlad tepes), R339X hg2, T301K |
[23] [48] |
gata1:GFP gata1:DsRed | [37, 49] |
| |||||
Myelopoiesis: GMPs | spi1/pu.1 | None | spi1:EGFP zpu.1:EGFP | [50, 51] | |
| |||||
mpx | None | mpx:GFP | [52] | ||
Myelopoiesis: Neutrophils, Macrophages, Monocytes | lyz | None | lyz:EGFP lyz:DsRed | [53] | |
mpeg1 | None | mpeg1:EGFP mpeg1:mCherry | [54] | ||
| |||||
rag1 | t26683, R797X | [55] | rag1:GFP | [56] | |
Lymphopoiesis | lck | None | lck:EGFP | [57] | |
ikzf1/ikaros | t24980, Q360X | [58] | ikzf1:GFP | [59] |