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. 2012 Jul 21;9(6):413–423. doi: 10.7150/ijms.4514

FIGURE 6.

FIGURE 6

The effect of miR-21 on ischemia-related cell injury in vitro. Cultured cardiac cell injury was induced by hypoxia for 4 h in a serum- and glucose-free medium followed by reoxygenation (H/R) for 3 h innormal culture medium. Cell apoptosis was determined by flow cytometry. A, miR-21 inhibitor, LNA-anti-miR-21 (30 nM) decreased, but pre-miR-21 (30 nM) increased miR-21 expression in cultured cardiac cells. n=5, *, p< 0.05 compared with the vehicle control. †, p< 0.05 compared with the LNA-anti-miR-21 group. B, hypoxia/reoxygenation resulted in an increase in apoptosis. Pre-miR-21 decreased the cardiac myocyte apoptosis. In contrast, cardiac myocyte apoptosis was exacerbated after treatment with LNA-anti-miR-21. n=5, *, p< 0.05 compared with the noninjured (without H/R) control. † , p< 0.05 compared with H/R treated with the vehicle control.