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. 2012 Jun 15;13(8):750–758. doi: 10.1038/embor.2012.88

Figure 2.

Figure 2

Kindlin 2 is required for maintaining the active form of β-catenin. (A) Stably expressed control or Kindlin 2 short hairpin RNA (shRNA) Hs578T cells were generated. Proteins were extracted for western blot (WB). (B) Control or Kindlin 2 small interfering RNA (siRNA)2 was transfected into 293T cells followed by WB. (C) MCF7–Flag or MCF7–Flag–Kindlin 2 stable cells were treated with cycloheximide (CHX, 25 μg/ml) for different time points as indicated, then proteins were extracted for WB. (D) GSK3β was transfected into the indicated stable cells, and then protein was extracted for WB. (E) GSK3β and control or Kindlin 2 siRNA2 were co-transfected into 293T cells followed by WB. (F) Cells (293T) were transfected with the indicated constructs, later protein was extracted for co-immunoprecipitation. (G) Flag, Flag–Kindlin 2 WT, or QW mutant was transfected into 293T cells, followed by WB. (H) WB analysis was performed for the indicated stable cells. IB, immunoblot; WT, wild-type.