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. 2012 Jun 15;13(8):750–758. doi: 10.1038/embor.2012.88

Figure 4.

Figure 4

Kindlin 2 promotes tumour cell invasion by enhancing Axin2 expression. (A) Lysates from MCF7–Flag–Kindlin 2 stable cells were used for chromatin immunoprecipitation (ChIP) with antibodies as indicated. Quantitative PCR (qPCR) was used to quantify the ChIP-enriched DNAs. The Axin2 open reading frame (ORF) region was used as a negative control. (B) The levels of Axin2 were detected by western blot (WB) and qPCR in indicated stable cells, and Kindlin 2 small interfering RNA (siRNA)2-treated 293T cells. (C) Cells (293T) were transfected with control or Kindlin 2 siRNAs plus LiCl treatment (20 mM, 6 h). Total RNAs were extracted for qPCR. (D) ChIP were performed using the indicated antibodies. (E,F) The levels of Snail were analysed by WB in indicated stable cells, Kindlin 2 siRNA2-treated 293T cells and Axin2 siRNA-treated MCF7–Flag–Kindlin 2 stable cells.