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. 2012 Jun 19;13(8):759–764. doi: 10.1038/embor.2012.90

Figure 2.

Figure 2

FUS overexpression fully rescues the Tau splicing phenotype. Hippocampal neurons (DIV2+7) were coinfected with lentivirus expressing the shLuc or shFUS#2 and HA-tagged shRNA-resistant FUS. (A) Immunoblots with the indicated antibodies. (B) Total RNA was analysed by quantitative PCR for total Tau and isoforms. Total Tau expression was normalized to YWHAZ. Tau isoform expression was normalized to total Tau levels. n=3, mean±s.e.m, analysis of variance with Bonferroni correction: **P<0.01. Note that samples from shLuc- and shFUS-transduced neurons were statistically indistinguishable on coexpression of shFUS-resistant FUS. FUS, Fused in sarcoma; HA, haemagglutinin; shFUS, short hairpin FUS; shLuc, short hairpin RNA targeting the luciferase transcript.