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. 2012 Jul 23;198(2):251–263. doi: 10.1083/jcb.201110101

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© 2012 Moutin et al.

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Figure 5. — Homer1a–mGlu5a interaction enables inhibition of NMDA currents. (A) NMDA-induced currents recorded in nontransfected neurons before (100% NMDA current at time 0 s) and during sequential perfusion of TAT-HomerW24Y and TAT-Homer1a proteins. Horizontal bars above each trace represent application of 100 µM NMDA. Each plot represents the mean ± SEM obtained from eight neurons (asterisks represent significant difference from NMDA current before TAT perfusion). (B) NMDA current density (top) and percentage of TAT-Homer1a–induced inhibition of NMDA current (bottom) measured in neurons transfected or not with mGlu-Cterm (to quench Homer1a) or mGlu-Cterm-P1124K (which cannot interact with Homer proteins). Each bar of the histogram represents the mean ± SEM obtained from eight neurons. *, P = 0.05. (C and D) Neurons transfected with shRNA₁ or shRNA₂ and GFP (transfection reporter) were used for either NMDA-induced current recording (C; same legend as in A) or immunostaining with an anti-mGlu5 antibody (D; boxed areas are magnified to the right of each image). (D, bottom) Quantification within a dendritic area of 5 µm × 15 µm. (E) Neurons transfected with shRNA₁, GFP (transfection reporter), and Myc-mGlu5a were immunolabeled with an anti-Myc antibody in nonpermeabilized conditions (top) or used to record NMDA-induced currents (bottom; same legend as in A). (F) Percentage of NMDA current in the presence of the mGlu5a antagonist (MPEP; 10 µM) before and after TAT-Homer1a perfusion. *, P = 0.05. (D and F) Each bar of histogram represents the mean ± SEM obtained from eight neurons.